This vector is a small, medium copy number, E.coli plasmid, 2172 bp in length.
The plasmid contain:
1 - the p15A replicon (ori) responsible for the replication of plasmid (source - plasmid pACYC184);
2 - the Cm(R) gene, coding for chloramphenicol acetyl transferase, that confers resistance to chloramphenicol (source - plasmid pACYC184);
3 - the region of E.coli lac operon containing a CAP protein binding site, promoter Plac, lac repressor binding site and the 5’-terminal part of the lacZ gene encoding the N-terminal fragment of beta-galactosidase. This fragment, whose synthesis can be induced by IPTG, is capable of intra-allelic (alpha) complementation with a defective form of beta-galactosidase encoded by the host (mutation delta(lacZ)M15). In the presence of IPTG, bacteria synthesize both fragments of the enzyme and form blue colonies on media with X-Gal. Insertion of DNA into the MCS located within the lacZ gene inactivates the N-terminal fragment of beta-galactosidase and abolishes alpha-complementation. Bacteria carrying recombinant plasmids therefore give rise to white colonies.
The map shows enzymes that cut pACYC-lacZ'1 once.
The plasmid is are fully compatible with those carrying the pMB1 and ColE1 replicons.